Cov txheej txheem tu vaj tse rau Pasteur pipettes suav nrog kev tu tam sim tom qab siv, xaiv kev tu raws li cov khoom siv, zam kev puas tsuaj rau lub cev, ua kom tsis muaj menyuam thiab tshuaj tua kab mob, qhuav cia, thiab ua raws li cov txheej txheem pov tseg kom raug. Cov ntsiab lus tshwj xeeb yog raws li hauv qab no:
Kev tu tam sim ntawd tom qab siv: Pasteur pipettes yuav tsum tau ntxuav tam sim ntawd tom qab txhua qhov kev siv los tiv thaiv kev sib kis - thiab xyuas kom meej tias tsis muaj cov kua seem nyob hauv phab ntsa sab hauv. Muab cov pipettes tso rau hauv lub phiab ntxuav, ntxiv ib qho tsim nyog tus neeg saib xyuas kev lom neeg, thiab yaug kom huv si nrog dej los yog dej deionized.
Xaiv txoj kev ntxuav raws li cov khoom siv
Yas Pasteur Pipettes: Cov no yuav tsum tau tsau rau hauv 70% acetic acid rau 15 feeb, tom qab ntawd yaug kom huv si kom huv si tshem tawm cov kua qaub.
Iav Pasteur Pipettes: Cov no yuav tsum tau yaug kom huv si txhawm rau tiv thaiv cov khoom seem. Lawv tsim nyog rau rov ua kom tsis muaj menyuam; Txawm li cas los xij, ib qho yuav tsum tau ua raws li cov txheej txheem ua kom tsis muaj menyuam tshwj xeeb- cua sov qhuav (160℃rau 2 teev) lossis siab -siab chav (121℃rau 15 feeb). Yooj yim so nrog cawv tsis txaus kom ua tau raws li qhov yuav tsum tau ua kom tsis muaj menyuam.
Zam kev puas tsuaj rau lub cev
Siv tau pov tseg, ua ntej-sterilized Pasteur pipettes yog tsim los siv ib zaug xwb. Kev siv lawv rov qab ua rau muaj kev pheej hmoo ntawm kev hla - paug; Yog li ntawd, lawv rov siv dua yog txwv nruj rau hauv cov ntawv thov kev sim siab, xws li kab lis kev cai ntawm tes lossis PCR.
Kev cuam tshuam ntev rau cov av ntub lossis siab - qhov kub thiab txias tuaj yeem ua rau cov straws yas deform los yog cov kab mob harbor. Tom qab siv, lawv yuav tsum tau muab cais tawm kom raug: cov khib nyiab sib kis yuav tsum tau siv autoclaving, thaum cov straws yas zoo tib yam yuav tsum tau rov ua dua kom txo qis ib puag ncig.
Ua kom tsis muaj menyuam thiab tua kab mob kom raug
Siab - Kub thiab Siab -Sterilization siab: Muab cov Pasteur pipettes uas yuav tsum tau ua kom tsis muaj menyuam rau hauv ib qho autoclave (siab-siab sterilizer). Ntxiv cov dej uas tsim nyog, kom ntseeg tau tias cov dej theem siab tshaj qhov ntsuas kab cim ntawm lub tshuab. Kaw lub qhov rooj, txuas lub hwj huam mov, thiab pib lub tshuab ua raws li cov txheej txheem kev khiav hauj lwm uas tau teev tseg hauv cov neeg siv phau ntawv. Tos kom tsis muaj menyuam kom tiav; thaum lub tshuab qhia tias cov qauv kev ua kom tsis muaj menyuam tau ua tiav, tshem tawm cov khoom siv hluav taws xob. Cia lub autoclave kom txias ua ntej tshem cov Pasteur pipettes.
Tshuaj Sterilization: Muab cov Pasteur pipettes uas yuav tsum tau ua kom tsis muaj menyuam rau hauv lub thawv uas muaj cov tshuaj sterilizing (xws li Corning Chlorocide, ethanol, thiab lwm yam). Txheeb xyuas qhov xav tau qhov xav tau, lub sijhawm sib cuag, thiab qhov ntsuas kub rau cov tshuaj tshwj xeeb uas siv. Muab cov Pasteur pipettes rau hauv cov tshuaj sterilant rau lub sijhawm teem tseg. Thaum ua tiav, tshem cov pipettes, yaug kom huv si, thiab qhuav tam sim ntawd.
Ultraviolet (UV) Sterilization: Muab cov Pasteur pipettes kom tsis muaj menyuam rau hauv UV sterilizer. Xyuas kom meej tias UV teeb nyob rau hauv lub sterilizer tsis zoo thiab tsis puas. Hloov mus rau UV sterilizer thiab ua haujlwm raws li cov txheej txheem teev tseg hauv phau ntawv siv. Tos rau lub voj voog kom tsis muaj menyuam kom tiav ua ntej tshem cov Pasteur pipettes.
Qhuav Cia
Tom qab ntxuav, Pasteur pipettes yuav tsum tau blotted qhuav nrog ib daim ntawv phuam thiab tom qab ntawd qhuav siv cov kwj ntawm huab cua.
Thaum qhuav thiab npaj, cov pipettes yuav muab ntim rau hauv cov hnab lim tsis muaj menyuam los yog qhwv hauv cov ntawv ntim thiab sau kom meej. Lawv yuav tsum tau muab cia rau hauv qhov chaw txias, qhuav - deb ntawm tshav ntuj ncaj qha - kom tsis txhob muaj kab mob los yog deformation.
Cov Txheej Txheem Kev Pov Hwm Pov Hwm Pov Hwm Pov Hwm Pov Hwm Pov Hwm Pov Hwm Pipette yuav tsum tsis txhob muab ntxuav kom rov siv dua. Tom qab siv, lawv yuav tsum tau kho raws li kev kho mob pov tseg thiab pov tseg kom raug raws li cov kev cai cuam tshuam los tiv thaiv ib puag ncig.